Zlecenie 7353253 - Zakup odczynników biologicznych i materiałów...

zamówienia Celem zamówienia jest zakup odczynników biologicznych i materiałów laboratoryjnych służących do realizacji badań w projekcie pt.„Rozwój terapii celowanych opartych na mechanizmie syntetycznej letalności w onkologii”. Przedmiot zamówienia 1. Set of 4 SMARCA4 siRNA – Human
• siRNA modified for greater specificity;
• aliquots of all 4 individual siRNAs targeting a single gene;
• Off-targets reduced by up to 90% compared to unmodified siRNA;
• sequence information provided with siRNA;
• unique dual-strand modification pattern to reduce off-targets caused by either strand;
Wielkość opakowania: 5 nmol Ilość: 1 CPV: 38951000-6

2. Pipetting tips
• for 384-channel pipetting and serial dilution using 16-channel magazine for semi-automatic 384-fold pipettor with minimal required space;
• net volume: 60 ul;
• 384 tips per tray;
Wielkość opakowania:24 trays Ilość: 3 CPV: 19520000-7

3. Set of 2 calibration plates
• calibration type: RNase P Instrument Verification Plate;
• dye: FAM;
• for 0,1 ml block;
• spectral calibration;
• 1 x 384-well plate and 1 x 96-well plate;
Wielkość opakowania:2 plates Ilość: 1 CPV: 19520000-7

4. 10% precast polyacrylamide gel
• well number (Vol): 15 (15 μl);
• for separation of polypeptides from 15–200 kD;
• Tis-glycine formulation, stain-free imaging of gels and blots in 1 min;
• can be used for denaturing electrophoresis with standard running buffer containing SDS;
• laemmli-like separation in the presence of SDS;
• proteins retain native conformation and activity in the absence SDS;
• run time as short as 15 min;
• shelf life: 12 months;
Wielkość opakowania:10 gels Ilość: 10 CPV: 33696600-1

5. Cell counting slides, dual-chamber slides for 2,400 counts
• counting parameters – range 5 × 104 – 1 × 107 cells/ml and 6 – 50 μm cell diameter;
• each slide can provide counts for 2 separate samples or dilutions;
• each chamber requires as little as 10 μl of suspended cells;
• both chambers can be loaded prior to insertion;
• volume counted in each chamber is 0.4 μl;
• can be used with or without trypan blue;
• for use with automated cell counters;
Wielkość opakowania: 40 x 30 slides Ilość: 1 CPV: 38437000-7

6. Actinomycin D
• from: Streptomyces species;
• purity (HPLC) > 95.0%;
• solubility: DMSO, ethanol;
• working concentrations: 0.01 to 2μg/m;
• used in cell culture as a selection agent;
Wielkość opakowania:5 mg Ilość: 1 CPV: 33696500-0