Zlecenie 7618256 - Zakup odczynników biologicznych 30 07 2020 C Odczynniki...

logicznych służących do realizacji badań w projekcie pt.„Rozwój terapii celowanych opartych na mechanizmie syntetycznej letalności w onkologii”. Przedmiot zamówienia 1. Anti-His-Tag antibody
• mouse monoclonal IgG1 (kappa light chain);
• raised against a His-tagged recombinant protein;
• for detection of fusion proteins encoded by polyhistidine expression vectors by Western Blotting (starting dilution
1:200, dilution range 1:100-1:1000) and Immunoprecipitation (1-2 µg per 100-500 µg of total protein (1 ml of cell lysate);
• supplied in in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin;
Wielkośc opakowania: 200 µg/ml Ilość: 1 CPV:24956000-0

2. Mouse IgG kappa binding protein conjugated to Horseradish Peroxidase (HRP)
• supplied in in 0.5 ml of PBS containing 40% glycerol, 1% stabilizer protein and < 0.01% thimerosal;
• suitable for binding to mouse IgGk light chain immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies;
Wielkośc opakowania: 200 µg/0.5 ml Ilość:1 CPV: 24956000-0

3. Anti-His-Tag antibody conjugated to Horseradish Peroxidase (HRP)
• mouse monoclonal IgG1 (kappa light chain);
• recommended for detection of fusion proteins encoded by polyhistidine expression vectors by WB and IP;
• supplied in n in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin;
Wielkośc opakowania: 200 µg/ml Ilość:1 CPV: 24956000-0

4. Cell viability reagent
• ready-to-use resazurin-based solution;
• functions as a cell health indicator by using the reducing power of living cells to quantitatively measure viability;
• is a non-toxic, cell-permeable compound that is blue in color and virtually non-fluorescent;
• can be used with various human and animal cell lines, bacteria, plant and fungi;
• format: 384-well plate, 96-well plate, cuvettes;
• incubation time: 1 to 4 hrs (up to 24 hrs for low cell numbers);
Wielkośc opakowania: 100 ml Ilość:1 CPV: 33696500-0