Zlecenie 7404563 - Zakup odczynników biologicznych 27 03 2020 A Odczynniki...

ogicznych służących do realizacji badań w projekcie pt. "Nowa generacja immunoterapii nowotworów oparta o aktywację odpowiedzi immunologicznej pacjentów". Przedmiot zamówienia 1. Calu-6 cell line
• human lung;
• model for anaplastic carcinoma;
• cel morphology: epithelial
• format:frozen;
Wielkość opakowania 1opakowanie Ilość: 1 CPV: 24956000-0

2. Perm wash buffer
• application: intracellular staining (flow cytometry);
• buffer: aqueous buffered solution containing saponin, fetal bovine serum and ≤ 0.09% sodium azide;
• concentration: 10X;
• used for the intracellular staining of post-translationally modified signaling proteins;
• can be used to permeabilize cells and to serve as an antibody diluent and cell wash buffer;
• intracellular phosphorylated signaling protein-specific antibodies;
Wielkość opakowania: 125 ml Ilość: 3 CPV: 33696500-0

3. Enhanced chemiluminescence (ECL) substrate for Western Blotting
• low-femtogram detection (as 10-15);
• low antibody consumption;
• ECL substrate with an extremely high signal intensity;
• contains: luminol/enhancer solution and peroxide solution;
• stable for 1 year at RT;
• working solution stable for at least 3 days;
• primary antibody dilution: 1:5.000-1:100.000 (for 1 mg/ml stock solution);
• secondary antibody dilution: 1:100.000-1:500.000 (for 1 mg/ml stock solution);
Wielkość opakowania: 2 x 50 ml Ilość: 2 CPV: 33696500-0

4. Phosphatase inhibitor tablets
• blend of phosphatase inhibitors;
• ready-to-use tablet;
• dissolves quickly in aqueous solutions (buffers), including buffers containing formalin (for formalin fixation);
• protects proteins against dephosphorylation;
• inhibits phosphatase classes such as acid and alkaline phosphatases, as well as serine/threonine (PP1, PP2A, and PP2B) and tyrosine protein phosphatases (PTP) in bacterial, mammalian, yeast, and plant extracts.
Wielkość opakowania: 20 tabletek Ilość: 1 CPV: 33696500-0

5. Cell lysis buffer
• enables extraction of cytoplasmic, membrane and nuclear proteins;
• compatible with many protein assays and immunoassays;
• used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures
• ready-to-use;
• form: liquid;
• contents: 25 mM Tris, HCl pH 7.6, 150 mM NaCl, 1%, NP-40, 1% sodium deoxycholate, 0.1% SDS ;
Wielkość opakowania: 250 ml Ilość: 1 CPV: 33696500-0

6. Phospho-IRF-3 (Ser396) rabbit monoclonal antibody
• reactivity: human, mouse
• isotype: Rabbit IgG
• supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% licerol and less than 0.02% sodium azide
• application: Western Blotting
• produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser396 of human IRF-3.
Wielkość opakowania: 100 ul Ilość: 1 CPV: 24956000-0

7. BCA protein assay kit
• two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) total protein concentration compared to a protein standard;
• assay range: 20-2,000µg/ml or 5-250µg/ml;
• detection method: colorimetric (562 nm);
• detect down to 5 µg/mL with enhanced protocol;
• contents: 2 x 500 ml BCA reagent A, 25 ml DCA reagent B and albumin standard ampules, 2mg/ml, 10 x 1 ml;
• can be stored at room temperaturę;
Wielkość opakowania: 1 kit Ilość: 1 CPV: 33141625-7

8. Purified mouse anti-human CD3 antibody
• isotype: Mouse IgG2a;
• reactivity: Human;
• quality control tested by immunofluorescent staining with flow cytometric analysis. 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.;
• endotoxin level: <0.01 EU/μg of the protein (<0.001 ng/μg of the protein);
• purified by affinity chromatography;
Wielkość opakowania: 5 mg Ilość: 1 CPV: 24956000-0