Zlecenie 12361478 - 1. 3T3‑J2 Cell Line • Subclone of mouse embryonic...

ke morphology. • Commonly used as a feeder layer to support growth of human epithelial cells. • Recommended culture conditions: 37 °C, 10% CO₂, DMEM supplemented with antibiotics, glutamine, and 10% bovine calf serum (not fetal serum). • Subculture when 60–80% confluent; suggested seeding density approx. 3.5–5 × 10³ cells/cm². • Approximate population doubling time: around 18 hours. • Biosafety level: BSL‑1; routinely tested for mycoplasma. • Cryopreservation: DMEM with serum and 10% DMSO; long‑term storage in liquid nitrogen. 1 ml x3 1. Viral concentration reagent • Increase the amount of lentiviral particles collected from cell culture supernatants using standard high-speed centrifugation • Incubation time: 1-hour at 4ᵒC • 1 ml sufficient to concentrate and purify virus from 1 liter of supernatant • Sterile, cell culture grade reagent, non-toxic to target

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FENG.01.01-IP.02-0095/23 - ONCO Prime: nowe możliwości personalizowanej terapii przeciwnowotworowej w oparciu o hodowlę komórek pierwotnych od pacjenta, charakteryzację omiczną i testy funkcjonalne