Zlecenie 7413774 - Zakup odczynników biologicznych i materiałów...

zamówienia Celem zamówienia jest zakup odczynników biologicznych i materiałów laboratoryjnych służących do realizacji badań w projekcie pt. "Nowa generacja immunoterapii nowotworów oparta o aktywację odpowiedzi immunologicznej pacjentów". Przedmiot zamówienia 1. Phospho-STING (Ser365) Rabbit monoclonal antibody
• reactivity: Mouse;
• sensitivity: endogenous;
• isotype: Rabbit IgG;
• application: Western Blot;
• buffer: 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA,
50% glycerol and less than 0.02% sodium azide ;
Wielkość opakowania: 100 µl Ilość: 1 CPV: 24956000-0

2. Phospho-STING Rabbit monoclonal antibody
• reactivity: Mouse;
• isotype: Rabbit IgG;
• application: Immunofluorescence (Immunocytochemistry);
• buffer: 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA,
50% glycerol and less than 0.02% sodium azide;
• recognizes endogenous levels of STING protein only when
phosphorylated at Ser365;
Wielkość opakowania: 100 µl Ilość: 1 CPV: 24956000-0

3. Rat anti-mouse CD8alpha antibody
• concentration:0.2 mg/ml;
• application: Flow cytometry;
• storage buffer: Aqueous buffered solution containing BSA
and ≤0.09% sodium azide;
• conjugated with UV-excitable dye with an excitation max of 348 nm
and emission max of 395 nm;
• excitation source :ultraviolet 355 nm;
Wielkość opakowania: 25 µg Ilość 1 CPV: 24956000-0

4. 96 well capmats for 2 ml plates
• material: EVA (Ethylene-vinyl acetate);
• free of detectable DNase, RNase, human DNA;
• non-pyrogenic;
• color: natural;
• resistant to DMSO;
• can be used in a temperature range between -20°C and + 60°C;
• square nap shape for sealing the 2 ml 96 well plates
• not pierceable, non-sterile;
Wielkość opakowania: 50 pieces Ilość: 8 CPV: 19520000-7

5. Mifepristone
• CAS: 84371-65-3;
• assay ≥ 98%;
• in glass bottle;
Wielkość opakowania: 100 mg Ilość: 1 CPV: 33696500-0

6. Human CYP2C19 recombinant enzyme
• enzyme type: P450;
• size: 0.5 nmole P450 in 0.5 ml;
• prepared from insect cells infected with baculovirus containing desired cDNAs;
• contains human oxidoreductase and cytochrome b5;
• cellular location: microsome;
Wielkość opakowania: 0,5 ml Ilość: 5 CPV: 24956000-0

7. Vacuum filter/storage bottle
• bottle capacity: 1000 ml;
• filter style: vacuum system ;
• funnel capacity: 1000 ml ;
• membrane material: Cellulose nitrate;
• membrane pore size : 0.2 µm;
• membrane area: 54.5 cm2 ;
• individually wrapped; with graduations;
• sterile, nonpyrogenic;
• neck diameter: 45 mm;
Wielkość opakowania: 1000 ml ILość: 1 CPV: 19520000-7

8. Permanent seal for microplates
• a permanent sealing tape for microplates when counting
Wielkość opakowania: 100 pieces Ilość: 1 CPV: 19520000-7

9. Lyophilized assay reagent to quantitatively measure the activity of Lucia luciferase and other coelenterazine-utilizing luciferases
• for use with Lucia luciferase reporter cell lines for fast and efficient real-time measurements directly from the cell culture media;
• contains the coelenterazine substrate for the luciferase reaction, which produces a light signal that is quantified using a luminometer and expressed as relative light units (RLU);
• one step reagent;
• no cell lysis required;
• reconstituted substrate is stable for up to a month;
• each pouch contains everything needed to prepare 25 ml of reagent allowing the preparation of 500 wells of a 96-well plate;
Wielkość opakowania: 5 pouches Ilość: 4 CPV: 33696500-0