Zlecenie 8076647 - Rozwój terapii celowanych opartych na mechanizmie...

Isotype: IgG • Unconjugated • Application: WB, ICC/IF, IHC-P, IP, ChIP assay • Reactivity: Human, Mouse • Buffer: 1XPBS, 20% Glycerol (pH7). CMIT/MIT, 3% added as a preservative. • Concentration: 0,50 mg/ml (+/-0,05 mg/ml) • Purified by antigen-affinity chromatography • Immunogen: Recombinant protein encompassing a sequence within the center region of human KAP1 100 ul x 2pKAP-1 Rabbit anti-Phospho KAP-1 antibody • Reactivity: Human, Mouse • Applications WB, IP, IHC, ICC, ICC-IF • Antibody Type: Polyclonal • Conjugate: Unconjugated • Format: Whole IgG • storage: Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide • concentration: 200 ug/ml (+/- 10 ug) • buffer: Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide 100 ul x 2Endonuclease, liquid •CAS no.: 9025-65-4; • purity ≥90%; • genetically engineered endonuclease from Serratia marcescens; • for removal of nucleic acids from recombinant proteins and protein fragments, for the reduction of viscosity in protein extracts; • completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2–5 bases in length; • formulation: ≥250 units/μL supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol; • derived From :Recombinant protein expressed in Escherichia coli; • the specific activity is 1.3×106 1.5×106 unit/mg protein; 25 KU x 1Phospho-H2AX (Ser139) cellular kit • direct detection of endogenous levels of H2AX in cells, only when phosphorylated at Ser139, quantitative detection; • phospho-H2AX (Ser139) detected in a sandwich assay format using 2 different specific antibodies, one labelled with Eu3+-Cryptate (donor) and the second with d2 (acceptor) – FRET; • assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate; • assay can be performed in a single plate used for plating, stimulation and detection; • possible cells viability and confluence monitored; • No washing steps required; • HTS designed (96 and 384 plate format), includes control lysate; 500 tests x 3Anti-Mus81 antibody (MTA30 2G10/3) • Mouse monoclonal • Isotype: IgG2a • Suitable for: WB, IP, Flow Cyt • Reactivity: Human • Storage buffer: PBS • Concentration: 100 μg at 1 mg/ml • Protein A purified 100 ug x 1BLM polyclonal antibody • Reactivity: Human; • Source: Rabbit; • Buffer: 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA and 50% glycerol; • Application: WB; • Purified by peptide affinity chromatography; 100 ul x 2WRN Mouse monoclonal antibody • Isotype: Mouse IgG1; • detects endogenous levels of total WRN protein; • supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA, 50% glycerol and less than 0.02% sodium azide; • application: WB; • species reactivity: Human, Mouse; • produced by immunizing animals with full length human recombinant WRN protein; 100 ul x 2ATR Antibody • detects endogenous levels of total ATR protein • Reactivity: Human, Monkey • source: Rabbit • Polyclonal antibody, • purified by protein A and peptide affinity chromatography • Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA and 50% glycerol. • application: Western blot (dilution 1:1000) 100 ul x 1pATR Phospho-ATR (Thr1989) Antibody • recognizes endogenous levels of ATR protein only when phosphorylated at Thr1989. • Reactivity: human • source: rabbit • Polyclonal antibody, • purified by protein A and peptide affinity chromatography • Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 μg/ml BSA and 50% glycerol. • application: Western blot (dilution 1:1000) 100 ul x 1